Y04603 Recombinant Human Wnt-3a (hFc Tag）

货号：Y04603

名称：Y04603 Recombinant Human Wnt-3a (hFc Tag）

种属：Human

规格：10μg/50μg/1mg

纯度：≥95%

内毒素： ≤100 EU/mg

表达系统：CHO

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(货号) Code

Y04603

(纯度) Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

(内毒素) Endotoxin

≤100 EU/mg by the LAL method.

(生物活性) Bioactivity

Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED₅₀ for this effect is ＜30 ng/mL.

(检索号) Accession

NP_149122

(别名) Alternative Names

MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

(种属) Species

Human

(表达系统) Expression System

CHO

(预测分子量大小) Mol. Weight

58.2 kDa

(表达区间) Expression Region

Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330)

(标签) Tag Info

hFc Tag

(剂型) Formulation

Lyophilized

(溶液) Buffer

Lyophilized from a 0.22 μm filtered solution containing PBS, pH7.4.

(规格) Size

10μg/50μg/1mg

(货期) Lead Time

Products are in stock, but custom products may require further consultation.

(复溶) Reconstitution

It is recommended to redissolve in sterile deionized water.

(运输) Shipping

The product is shipped with blue ice.

(存储) Storage & Stability

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

数据

SDS-PAGE

Y04603 Recombinant Human Wnt-3a(hFc Tag).jpg

Recombinant Human Wnt-3a (Fc Tag) (Cat. No. Y04603) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04603 SDS-PAGE.

Bioactivity

Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 5 ng/mL BMP2. The ED₅₀ for this effect is ＜30 ng/mL. The bioactivity of Recombinant Human Wnt-3a (Fc Tag) (Cat. No. Y04603) was higher than other competing products.

Bioactivity

Highly consistent bioactivity between different production batches of Recombinant Human Wnt-3a (Fc Tag) (Cat. No. Y04063).